Scotland international McLaughlin, 33, has come under increasing fire from Rangers supporters after shipping eight goals in his last two games.
Particularly against Celtic, the goalkeeper was at fault for all four goals and there have been grumbles from within the Ibrox support that they want to see the player taken out the team.
But under pressure manager Giovanni van Bronckhorst has had the decision made for him after Jon McLaughlin did not turn out in Rangers training ahead of the Champions League clash.
Speaking ahead of the game with Napoli, the Rangers manager confirmed that Jon McLaughlin had picked up a knock against Ajax and that Allan McGregor would start against the Italians.
“McLaughlin has picked up an injury and will not be involved. It is different for Allan this season, he has still been the same in training,” said van Bronckhorst [Rangers].
“I have every faith in him tomorrow with the experience he has, no doubt in my mind that he will perform tomorrow.”
Jon McLaughlin out but Tavernier fit for Rangers
The Rangers manager also confirmed that Ibrox captain James Tavernier is fit enough for the Napoli clash and welcomed the one week break in helping the player recover physically.
Tavernier, 30, came off the park against the Dutch champions at halftime with Rangers 3-0 down on the night.
“Tavernier is doing better than the last few weeks, the weekend allowed him more time to recover,” said van Bronckhorst.
“Physically, he should be in a much better place than in the match against Ajax.”
Rangers legend Allan McGregor, 40, has huge experience for the Ibrox club on big European nights and will make only his second appearance of the season against Napoli.
The veteran stopper’s only appearance to date came in the 3-1 League Cup win over Queen of the South.
Meanwhile, the SFA’s latest statement to Scottish football means Rangers fans can expect to look out their scarves this weekend.